Skip to main content Skip to navigation
Hamburger Menu
Search icon
Order lookup icon Order lookup icon
Order Lookup
Country Selector Country Selector
United States (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      PE Rat Anti-Mouse Vβ 2 TCR
      Alert icon
      This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
      PE Rat Anti-Mouse Vβ 2 TCR
      Two-color analysis of the expression of Vβ 2 TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with PE-conjugated B20.6, FITC-conjugated RM4-5 (anti-CD4, Cat. No. 553046/553047), and FITC-conjugated 53-6.7 (anti-CD8a, Cat. No. 553030/553031) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
      PE Rat Anti-Mouse Vβ 2 TCR
      Two-color analysis of the expression of Vβ 2 TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with PE-conjugated B20.6, FITC-conjugated RM4-5 (anti-CD4, Cat. No. 553046/553047), and FITC-conjugated 53-6.7 (anti-CD8a, Cat. No. 553030/553031) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      Mouse (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
      Mouse Cytotoxic T-Cell Clone Soluble αβ TCR
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_394754
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      Show More blue down arrow Show Less blue up arrow
      553281 Rev. 9
      Antibody Details
      Down Arrow Up Arrow
      B20.6

      The B20.6 monoclonal antibody specifically recognizes the Vβ 2 T-cell Receptor of all strains tested. Vβ 2 TCR-bearing T lymphocytes are clonally eliminated in mice expressing I-E and exposed neonatally to milk-borne BALB/cV virus, a highly tumorigenic strain of MMTV. Exposure of adult mice to BALB/cV virus results in expansion of Vβ 2 TCR-bearing T cells, which is partially dependent upon presentation by I-E.

      This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      553281 Rev. 9
      Format Details
      Down Arrow Up Arrow
      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      553281 Rev.9
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "553281" on CiteAb

      Development References (3)

      1. Grégoire C, Rebaï N, Schweisguth F, et al. Engineered secreted T-cell receptor alpha beta heterodimers.. Proc Natl Acad Sci USA. 1991; 88(18):8077-81. (Clone-specific). View Reference
      2. Hodes RJ, Novick MB, Palmer LD, Knepper JE. Association of a V beta 2-specific superantigen with a tumorigenic milk-borne mouse mammary tumor virus. J Immunol. 1993; 150(4):1422-1428. (Biology). View Reference
      3. Necker A, Rebai N, Matthes M, et al. Monoclonal antibodies raised against engineered soluble mouse T cell receptors and specific for V alpha 8-, V beta 2- or V beta 10-bearing T cells. Eur J Immunol. 1991; 21(12):3035-3040. (Immunogen). View Reference
      553281 Rev. 9

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.