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      FITC Rat Anti-Mouse Vα 11.1, 11.2[b,d] TCR
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      This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
      FITC Rat Anti-Mouse Vα 11.1, 11.2[b,d] TCR
      Two-color analysis of the expression of Vα 11.1, 11.2[b,d] TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with FITC-conjugated RR8-1, PE-conjugated RM4-5 (anti-CD4, Cat. No. 553048/553049), and PE-conjugated 53-6.7 (anti-CD8a, Cat. No. 553032/553033) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
      FITC Rat Anti-Mouse Vα 11.1, 11.2[b,d] TCR
      Two-color analysis of the expression of Vα 11.1, 11.2[b,d] TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with FITC-conjugated RR8-1, PE-conjugated RM4-5 (anti-CD4, Cat. No. 553048/553049), and PE-conjugated 53-6.7 (anti-CD8a, Cat. No. 553032/553033) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
      Product Details
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      BD Pharmingen™
      Mouse (QC Testing)
      Rat IgG2b, κ
      Mouse T-Cell Clone B10
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      AB_394717
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      553222 Rev. 9
      Antibody Details
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      RR8-1

      The RR8-1 monoclonal antibody specifically recognizes the Vα 11.1 and Vα 11.2, but not Vα 11.3 T-cell Receptors (TCR) of mice having the b (e.g., C57BL) and d (e.g., DBA/1, DBA/2, NZW) haplotypes of Tcra gene complex. RR8-1 antibody does not react with strains having the a (e.g., A, AKR, BALB/c, CBA, C3H/He) or c (e.g., NZB, SJL, SWR, NOD) Tcra haplotypes. Plate-bound RR8-1 antibody activates Vα 11.1, 11.2[b,d] TCR-bearing T lymphocytes.

      This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      553222 Rev. 9
      Format Details
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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      FITC
      Blue 488 nm
      494 nm
      518 nm
      553222 Rev.9
      Citations & References
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      View product citations for antibody "553222" on CiteAb

      Development References (1)

      1. Jameson SC, Nakajima PB, Brooks JL, Heath W, Kanagawa O, Gascoigne NR. The T cell receptor V alpha 11 gene family. Analysis of allelic sequence polymorphism and demonstration of J alpha region-dependent recognition by allele-specific antibodies. J Immunol. 1991; 147(9):3185-3193. (Immunogen). View Reference
      553222 Rev. 9

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.