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      FITC Rat Anti-Mouse Vβ 7 T-Cell Receptor
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      This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
      FITC Rat Anti-Mouse Vβ 7 T-Cell Receptor
      Two-color analysis of the expression of Vβ 7 TCR on peripheral T lymphocytes. SJL lymph node cells were simultaneously stained with FITC-conjugated TR310, PE-conjugated RM4-5 (anti-CD4, Cat. No. 553048/553049), and PE-conjugated 53-6.7 (anti-CD8a, Cat. No. 553032/553033) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
      FITC Rat Anti-Mouse Vβ 7 T-Cell Receptor
      Two-color analysis of the expression of Vβ 7 TCR on peripheral T lymphocytes. SJL lymph node cells were simultaneously stained with FITC-conjugated TR310, PE-conjugated RM4-5 (anti-CD4, Cat. No. 553048/553049), and PE-conjugated 53-6.7 (anti-CD8a, Cat. No. 553032/553033) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
      Product Details
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      BD Pharmingen™
      Mouse (QC Testing)
      Rat F344, also known as Fischer, CDF IgG2b, κ
      AKR/Cum Mouse spontaneous T Lymphoma Cell Line TK1
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      AB_394712
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      553215 Rev. 12
      Antibody Details
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      TR310

      The TR310 monoclonal antibody specifically recognizes the Vβ 7 T-Cell Receptor (TCR) of strains having the a (e.g., C57BR, C57L, SJL), b (e.g., A, BALB/c, CBA/Ca, C3H/He, C57BL, DBA/1), and c (e.g., RIII) haplotypes of the Tcrb gene complex. Vβ 7 is among the predominant TCRs expressed by NK-T cells of C57BL/6 mice. Vβ 7 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigens encoded by Mtv-7 (Mls-1[a], Mls[a]) endogenous proviruses (e.g., AKR, CBA/J, C58, DBA/2, NZB). Activation or elimination of Vβ 7 TCR-expressing T cells by these determinants is partially dependent upon presentation by I-E. Mtv-43 and/or exogenous MMTV-SW superantigens also cause incomplete elimination of Vβ 7 TCR-bearing T cells.

      553215 Rev. 12
      Format Details
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      553215 Rev.12
      Citations & References
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      View product citations for antibody "553215" on CiteAb

      Development References (4)

      1. Bendelac A. Mouse NK1+ T cells. Curr Opin Immunol. 1995; 7(3):367-374. (Biology). View Reference
      2. Hodes RJ, Abe R. Mouse endogenous superantigens: Ms and Mls-like determinants encoded by mouse retroviruses.. Curr Protoc Immunol. 2001; Appendix 1:Appendix 1F. (Biology). View Reference
      3. Okada CY, Holzmann B, Guidos C, Palmer E, Weissman IL. Characterization of a rat monoclonal antibody specific for a determinant encoded by the V beta 7 gene segment. Depletion of V beta 7+ T cells in mice with Mls-1a haplotype. J Immunol. 1990; 144(9):3473-3477. (Immunogen). View Reference
      4. Shinohara K, Ikarashi Y, Maruoka H, et al. Functional and phenotypical characteristics of hepatic NK-like T cells in NK1.1-positive and -negative mouse strains. Eur J Immunol. 1999; 29(6):1871-1878. (Biology). View Reference
      View All (4) View Less
      553215 Rev. 12

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.