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      Purified Rat Anti-Mouse F4/80-Like Receptor
      Purified Rat Anti-Mouse F4/80-Like Receptor
      Three-analysis of the expression of FIRE on bone-marrow leukocytes. BALB/c bone-marrow leukocytes were stained with either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553297, left panels) or purified mAb 6F12 (right panels), in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142), followed by biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894), then Streptavidin-APC (Cat. No. 554067). Myeloid cells were identified by staining with FITC-conjugated anti-mouse CD11b mAb M1/70 (Cat. No. 557396/553310, upper panels)and dendritic cells were identified with PE-conjugated anti-mouse Cd11c mAb HL3 (Cat. No. 557401/553802, bottom panels). The right panels demonstrate that the hematopoietic precursors which express FIRE protein are CD11b[intermediate]CD11c-. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
      Purified Rat Anti-Mouse F4/80-Like Receptor
      Three-analysis of the expression of FIRE on bone-marrow leukocytes. BALB/c bone-marrow leukocytes were stained with either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553297, left panels) or purified mAb 6F12 (right panels), in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142), followed by biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894), then Streptavidin-APC (Cat. No. 554067). Myeloid cells were identified by staining with FITC-conjugated anti-mouse CD11b mAb M1/70 (Cat. No. 557396/553310, upper panels)and dendritic cells were identified with PE-conjugated anti-mouse Cd11c mAb HL3 (Cat. No. 557401/553802, bottom panels). The right panels demonstrate that the hematopoietic precursors which express FIRE protein are CD11b[intermediate]CD11c-. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
      Product Details
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      BD Pharmingen™
      Fire; Emr4; EGF-like module receptor 4; D17Ertd479e; Egf-tm7
      Mouse (QC Testing)
      Rat IgG2a, κ
      CHO cells expressing recombinant FIRE fusion protein
      Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Reported), Immunoprecipitation, Western blot (Not Recommended)
      0.5 mg/ml
      52614
      AB_394533
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Recommended Assay Procedures

      For flow cytometry of leukocyte suspensions, we recommend that Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142) be used. Since this antigen is expressed at low density on myeloid cells, it may be desirable to amplify staining by using a biotinylated second-step antibody followed by a "bright" third-step reagent, such as Streptavidin-APC (Cat. No. 554067). If Mouse BD Fc Block™ antibody is used, it is important that the second-step anti-rat IgG does not react with 2.4G2 mAb (rat IgG2b, κ); we recommend biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894).

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      552958 Rev. 2
      Antibody Details
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      6F12

      The 6F12 antibody reacts with a 7-transmembrane-domain protein, which is similar to the F4/80 macrophage antigen of the EGF-TM7 protein family and is encoded by the Emr4 gene. The FIRE protein is expressed on myeloid cells with a denditic cell (DC) developmental potential, including subsets of DC and macrophages in the spleen and lymph nodes, most resident peritoneal macrophages, many peripheral blood monocytes, and a subpopulation of bone-marrow myeloid-cell progenitors. The protein is not detected on peripheral T and B lymphocytes, and it is down-regulated on thioglycollate-elicited peritoneal macrophages and on dendritic cells activated by GM-CSF, IFN-γ, anti-CD40, and LPS. Using soluble biotinylated fusion protein, a FIRE ligand was detected on a mouse IgG+ B lymphoma cell line (A20), but not on myeloid, fibroblast, or T-cell lines, suggesting that the FIRE protein may be involved in immunoregulatory interactions between antigen-presenting cells and B lymphocytes.

      552958 Rev. 2
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      552958 Rev.2
      Citations & References
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      View product citations for antibody "552958" on CiteAb

      Development References (2)

      1. Caminschi I, Lucas KM, O'Keeffe MA, et al. Molecular cloning of F4/80-like-receptor, a seven-span membrane protein expressed differentially by dendritic cell and monocyte-macrophage subpopulations. J Immunol. 2001; 167(7):3570-3576. (Immunogen: Immunohistochemistry, Immunoprecipitation, Western blot). View Reference
      2. Stacey M, Chang GW, Sanos SL, et al. EMR4, a novel epidermal growth factor (EGF)-TM7 molecule up-regulated in activated mouse macrophages, binds to a putative cellular ligand on B lymphoma cell line A20. J Biol Chem. 2002; 277(32):29283. (Biology). View Reference
      552958 Rev. 2

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.