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      Biotin Rat Anti-Human CXCR5 (CD185)
      Biotin Rat Anti-Human CXCR5 (CD185)
      Detection of CXCR5 (CD185)  expression on human peripheral lymphocytes by Biotin Rat Anti-Human CXCR5 (CD185). Human peripheral blood mononuclear cells were stained with Biotin Rat Anti-Human CXCR5 (CD185)(Cat. No. 552118) and FITC Mouse Anti-Human CD3 (Cat. No. 555339; Left Panel) or FITC Mouse Anti-Human CD19 (Cat. No. 555412; Right Panel). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using Biotin Rat IgG2b, κ Isotype Control (Cat. No. 555847) and FITC-conjugated mouse IgG2a, κ Isotype Control (Cat. No. 555573) or FITC-conjugated mouse IgG1, κ Isotype Control (Cat. No. 555748). The quadrant markers for the bivariate dot plots were set based on the isotype controls.  
      Biotin Rat Anti-Human CXCR5 (CD185)
      Detection of CXCR5 (CD185)  expression on human peripheral lymphocytes by Biotin Rat Anti-Human CXCR5 (CD185). Human peripheral blood mononuclear cells were stained with Biotin Rat Anti-Human CXCR5 (CD185)(Cat. No. 552118) and FITC Mouse Anti-Human CD3 (Cat. No. 555339; Left Panel) or FITC Mouse Anti-Human CD19 (Cat. No. 555412; Right Panel). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using Biotin Rat IgG2b, κ Isotype Control (Cat. No. 555847) and FITC-conjugated mouse IgG2a, κ Isotype Control (Cat. No. 555573) or FITC-conjugated mouse IgG1, κ Isotype Control (Cat. No. 555748). The quadrant markers for the bivariate dot plots were set based on the isotype controls.  
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      BD Pharmingen™
      CD185; Chemokine C-X-C motif receptor 5; BLR1; MDR-15
      Human (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2b, κ
      Human CXCR5 (CD185)
      Flow cytometry (Routinely Tested)
      20 µl/test
      643
      AB_394337
      Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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      Recommended Assay Procedures

      Biotin Rat Anti-Human CXCR5 (CD185) is effective for indirect immunofluorescence staining of human cells for flow cytometric analysis using 20 µl/10^6 cells. Combined with our Streptavidin-phycoerythrin (Cat. No. 554061), this antibody can be used for immunofluorescent staining and flow cytometric analyses of human leukocytes and cell lines expressing CXCR5 (see figure). For chemokine receptors expressed at low frequencies on the surface of cells, a 3-step staining procedure is recommended.

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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      552118 Rev. 5
      Antibody Details
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      RF8B2

      The RF8B2 monoclonal antibody specifically binds to the human CXC chemokine receptor, CXCR5. CXCR5 (also known as CD185, BLR-1 NLR and MDR15), a seven transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokine, CXCL13/BLC/BCA-1. In peripheral blood, CXCR5 expression is restricted to B lymphocytes and a small subset of CD4+ and CD8+ lymphocytes. The restricted expression pattern of CXCR5 on B cells and follicular T helper cells (Tfh) suggests that this receptor functions as a regulator of B and T cell migration. Stimulation of T cells with anti-CD3 monoclonal antibody leads to the down-regulation of CXCR5.

      552118 Rev. 5
      Format Details
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      Biotin
      Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
      Biotin
      552118 Rev.5
      Citations & References
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      View product citations for antibody "552118" on CiteAb

      Development References (6)

      1. Barella L, Loetscher M, Tobler A, Baggiolini M, Moser B. Sequence variation of a novel heptahelical leucocyte receptor through alternative transcript formation. Biochem J. 1995; 309(3):773-779. (Biology). View Reference
      2. Dobner T, Wolf I, Emrich T, Lipp M. Differentiation-specific expression of a novel G protein-coupled receptor from Burkitt's lymphoma. Eur J Immunol. 1992; 22(11):2795-2799. (Biology). View Reference
      3. Forster R, Emrich T, Kremmer E, Lipp M. Expression of the G-protein--coupled receptor BLR1 defines mature, recirculating B cells and a subset of T-helper memory cells. Blood. 1994; 84(3):830-840. (Immunogen). View Reference
      4. Gunn MD, Ngo VN, Ansel KM, Ekland EH, Cyster JG, Williams LT. A B-cell-homing chemokine made in lymphoid follicles activates Burkitt's lymphoma receptor-1. Nature. 1998; 391(6669):799-803. (Biology). View Reference
      5. Kouba M, Vanetti M, Wang X, Schafer M, Hollt V. Cloning of a novel putative G-protein-coupled receptor (NLR) which is expressed in neuronal and lymphatic tissue. FEBS Lett. 1993; 321(2-3):173-178. (Biology). View Reference
      6. Legler DF, Loetscher M, Roos RS, Clark-Lewis I, Baggiolini M, Moser B. B cell-attracting chemokine 1, a human CXC chemokine expressed in lymphoid tissues, selectively attracts B lymphocytes via BLR1/CXCR5. J Exp Med. 1998; 187(4):655-660. (Biology). View Reference
      View All (6) View Less
      552118 Rev. 5

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.