BD Pharmingen™ Purified Mouse Anti-Human CD247 with Control

Western blot:  Please refer to http://www.bdbiosciences.com/support/resources/cell_biology/index.jsp

Jurkat control lysate [50 µg (1 µg/µl) is provided as a Western blot positive control (Comp. 51-16526N; store lysate at -20°C). Additional control lysate (Cat. No. 611451) is sold separately as a ready-to-use western blot control.

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
5. An isotype control should be used at the same concentration as the antibody of interest.
6. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.