Flow cytometric analysis of Human Blood Group A expression on human red blood cells. Human red blood cells were stained with either FITC Mouse IgG3, κ Isotype Control (Cat. No. 555578; dashed line histogram) or PE Mouse Anti-Human Blood Group A antibody (Cat. No. 550807; solid line histogram). The fluorescence histogram showing Human Blood Group A expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of erythrocytes.
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Flow cytometric analysis of Human Blood Group A expression on human red blood cells. Human red blood cells were stained with either FITC Mouse IgG3, κ Isotype Control (Cat. No. 555578; dashed line histogram) or PE Mouse Anti-Human Blood Group A antibody (Cat. No. 550807; solid line histogram). The fluorescence histogram showing Human Blood Group A expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of erythrocytes.
Product Details
BD Pharmingen™
Human (QC Testing)
Mouse IgG3, κ
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_393892
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
FITC Mouse IgG3, κ Isotype Control RUO
Size
100 Tests
Cat No.
555578
550807 Rev. 2
Antibody Details
NaM87-1F6
The NaM87-1F6 monoclonal antibody specifically binds to blood group A expressed on human erythrocytes. Clone NaM87-1F6 induces agglutination of human group A red blood cells. It does not react with human group B, or group O red blood cells.
550807 Rev. 2
Format Details
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
550807 Rev.2
Citations & References
View product citations for antibody "550807" on CiteAb
Development References (3)
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Blanchard D, Bruneau V, Bernard D, et al. Flow cytometry analysis of dual red blood cell populations after bone marrow transplantation. Br J Haematol. 1995; 89(4):741-747. (Clone-specific: Flow cytometry). View Reference
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David B, Bernard D, Navenot JM, Muller JY, Blanchard D. Flow cytometric monitoring of red blood cell chimerism after bone marrow transplantation. Transfus Med. 1999; 9(3):209-217. (Clone-specific: Flow cytometry).
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Walker RH, ed. Technical Manual of the American Association of Blood Banks. Arlington VA: 1990.
550807 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.