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PE Mouse Anti-Human CD11b
Product Details
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BD™
MAC-1A; Mac-1; ITGAM; Integrin alpha M; CR3A; CR-3 alpha; Mo1; SLEB6
Human
Mouse BALB/c IgG2a, κ
Human peripheral blood T lymphocytes
Flow cytometry
50 μg/mL
20 μL
3684
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

The monoclonal antibody is supplied as 100 μg purified immunoglobulin in 2.0 mL (50 μg/mL) of phosphate-buffered saline (PBS). The PE conjugate is supplied as 100 μg in 2.0 mL (50 μg/mL). The APC conjugate is supplied as 25 μg in 0.5 mL (50 μg/mL). PBS contains gelatin and 0.1% sodium azide.

347557 Rev. 1
Antibody Details
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D12

The CD11b antibody, clone D12, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with peripheral blood T lymphocytes.

The CD11b antibody recognizes a human leucocyte antigen that is the C3bi complement receptor (CR3). CD11b is specific for the 165-kilodalton (kDa) α subunit of the CD11b/CD18 antigen heterodimer. The 95-kDa CD18 antigen, the β chain of leucocyte function–associated antigen-1 (LFA-1β), is common to the CD11a/CD18 (LFA-1) and CD11c/CD18 antigen heterodimers.

347557 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
347557 Rev.1
Citations & References
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View product citations for antibody "347557" on CiteAb

Development References (10)

  1. Lanier LL, Phillips JH. A map of the cell surface antigens expressed on resting and activated human natural killer cells. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:157-170.
  2. Bernstein ID, Self S. Joint report of the Myeloid Section of the Second International Workshop on Human Leukocyte Differentiation Antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human Myeloid and Hematopoietic Cells. New York, NY: Springer-Verlag; 1986:1-25.
  3. Bray RA, Gottschalk LR, Landay AL, Gebel HM. Differential surface marker expression in patients with CD-16+ lymphoproliferative disorders: in vivo model for NK differentiation.. Hum Immunol. 1987; 19(2):105-15. (Biology). View Reference
  4. Gebel HM, Kaizer H, Landay AL. Characterization of circulating suppressor T lymphocytes in bone marrow transplant recipients.. Transplantation. 1987; 43(2):258-63. (Biology). View Reference
  5. Landay A, Gartland GL, Clement LT. Characterization of a phenotypically distinct subpopulation of Leu-2+ cells that suppresses T cell proliferative responses.. J Immunol. 1983; 131(6):2757-61. (Biology). View Reference
  6. Landay A, Gartland GL, Clement LT. Characterization of a phenotypically distinct subpopulation of Leu-2+ cells that suppresses T cell proliferative responses.. J Immunol. 1983; 131(6):2757-61. (Biology). View Reference
  7. Patarroyo M, Makgoba MW. Leucocyte adhesion to cells. Molecular basis, physiological relevance, and abnormalities.. Scand J Immunol. 1989; 30(2):129-64. (Biology). View Reference
  8. Repo H, Jansson SE, Leirisalo-Repo M. Anticoagulant selection influences flow cytometric determination of CD11b upregulation in vivo and ex vivo.. J Immunol Methods. 1995; 185(1):65-79. (Biology). View Reference
  9. Ross GD, Cain JA, Lachmann PJ. Membrane complement receptor type three (CR3) has lectin-like properties analogous to bovine conglutinin as functions as a receptor for zymosan and rabbit erythrocytes as well as a receptor for iC3b.. J Immunol. 1985; 134(5):3307-15. (Biology). View Reference
  10. Shalekoff S, Page-Shipp L, Tiemessen CT. Effects of anticoagulants and temperature on expression of activation markers CD11b and HLA-DR on human leukocytes.. Clin Diagn Lab Immunol. 1998; 5(5):695-702. (Biology). View Reference
View All (10) View Less
347557 Rev. 1

 

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