PE Mouse Anti-Human CD14

BD™ PE Mouse Anti-Human CD14

Name: PE Mouse Anti-Human CD14
Brand: BD™
SKU: 347497

Clone MφP9 (also known as MφP-9)

(RUO (GMP))

Spectrum Viewer

Protocol Library

Scientific Resources

Product Details

Brand: BD™

Alternative Name: LPS receptor; LPS-R; Myeloid cell-specific leucine-rich glycoprotein

Reactivity: Human

Isotype: Mouse BALB/c IgG2b, κ

Immunogen: Human Monocytes

Application: Flow cytometry

Concentration: 50 μg/mL

Vol. Per Test: 20 μL

Workshop Number: I M35; II M67; III M337; IV M301

Entrez Gene ID: 929

RRID: AB_400312

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

347497 Rev. 1

Antibody Details

MφP9

The CD14 antibody, clone MφP9, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells from BALB/c mice immunized with peripheral blood monocytes from a patient with rheumatoid arthritis.

The CD14 antibody binds specifically to the 53–55 kilodalton (kDa) glycosylphosphatidylinositol (GPI)–anchored single-chain glycoprotein, CD14, also known as the LPS receptor or LPS-R.

347497 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

347497 Rev.1

Citations & References

View product citations for antibody "347497" on CiteAb

Development References (11)

Banchereau J, Briere F, Caux C. Immunobiology of dendritic cells. Annu Rev Immunol. 2000; 18:767-811. (Biology). View Reference
Bernstein ID, Self S. Joint report of the Myeloid Section of the Second International Workshop on Human Leukocyte Differentiation Antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human Myeloid and Hematopoietic Cells. New York, NY: Springer-Verlag; 1986:1-25.
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Chomarat P, Dantin C, Bennett L, Banchereau J, Palucka AK. TNF skews monocyte differentiation from macrophages to dendritic cells. J Immunol. 2003; 171:2262–2269. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Dimitriu-Bona A, Burmester GR, Waters SJ, Winchester RJ. Human mononuclear phagocyte differentiation antigens. I. Patterns of antigenic expression on the surface of human monocytes and macrophages defined by monoclonal antibodies. J Immunol. 1983; 130(1):145-152. (Biology). View Reference
Goyert SM, Ferrero E. Biochemical analysis of myeloid antigens and cDNA expression of gp55 (CD14). In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:613-619.
Herrmann F, Komischke B, Odenwald E, Ludwig W. Use of monoclonal antibodies as a diagnostic tool in human leukemia, I: Acute myeloid leukemia and acute phase of chronic myeloid leukemia. Blut. 1983; 47:157-163. (Biology).
Jayaram Y, Hogg N. Surface expression of CD14 molecules on human neutrophils. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:796-797.
Li G, Kim YJ, Mantel C, Broxmeyer HE. P-selectin enhances generation of CD14+CD16+ dentritic-like cells and inhibits macrophage maturation from human peripheral blood monocytes. J Immunol. 2003; 171:669–677. (Biology).
Scherberich JE, Nockher WA. CD14++ monocytes, CD14+/CD16+ subset and soluble CD14 as biological markers of inflammatory systemic diseases and monitoring immunosuppressive therapy. Annu Rev Immunol. 1999; 37:209–213. (Biology).

View All (11)

347497 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.