Anti-EpCAM PE

BD™ Anti-EpCAM PE

Name: Anti-EpCAM PE
Brand: BD™
SKU: 347211

Clone EBA-1

(ASR)

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Product Details

Brand: BD™

Alternative Name: EPCAM; EGP; ESA; GA733-2; hEGP-2; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1

Reactivity: Human

Isotype: Mouse BALB/c IgG1, λ

Immunogen: Breast carcinoma–associated mucin BCA-225

Application: Flow cytometry

Concentration: 3 μg/mL

Vol. Per Test: 20 μL

Entrez Gene ID: 4072

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: ASR

Preparation And Storage

Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

347211 Rev. 1

Antibody Details

EBA-1

Anti-EpCAM, clone EBA-1, is derived from the fusion of SP2 myeloma cells with spleen cells from BALB/c mice immunized with macromolecular aggregates containing breast carcinoma–associated mucin BCA-225.

Anti-EpCAM is directed against a human epithelial adhesion molecule (EpCAM) that has been identified as a ~40-kilodalton (kd) protein in Western blotting. This antigen is also known as epithelial surface antigen (ESA) and epithelial glycoprotein 2 (EGP-2).

347211 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

347211 Rev.1

Citations & References

View product citations for antibody "347211" on CiteAb

Development References (3)

De Leij L, Helrich W, Stein R, Mattes MJ. SCLC-cluster-2 antibodies detect the pancarcinoma/epithelial glycoprotein EGP-2. Int J Cancer. 1994; 8:60-63. (Biology). View Reference
Stahel RA, Gilks WR, Lehmann HP, Schenker T. Third International Workshop on Lung Tumor and Differentiation Antigens: overview of the results of the central data analysis. Int J Cancer. 1994; 8:6-26. (Biology). View Reference
Yemul S, Leon Ja, Pozniakoff T, Esser PD, Estabrook A. Radioimmunoimaging of human breast carcinoma xenografts in nude mouse model with 111In-labeled new monoclonal antibody EBA-1 and F(ab')2 fragments. Nucl Med Biol. 1993; 20:325-335. (Biology). View Reference

347211 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For In Vitro Diagnostic Use.

23-22942-00

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Documents are subject to revision without notice. Please verify you have the correct revision of the document, and always refer back to BD's eIFU website for the latest and most up to date information.