PE Mouse Anti-Human CD23

BD™ PE Mouse Anti-Human CD23

Name: PE Mouse Anti-Human CD23
Brand: BD™
SKU: 341007

Clone EBVCS-5 (also known as EBVCS 5)

(RUO (GMP))

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Product Details

Brand: BD™

Alternative Name: CD23A; Fc-epsilon-RII; FCER2; FCE2; IGEBF; BLAST-2; CLEC4J; Leu-20

Reactivity: Human

Isotype: Mouse BALB.B IgG1, κ

Immunogen: Epstein-Barr Virus Transformed Cell Line

Application: Flow cytometry

Concentration: 12.5 μg/mL

Vol. Per Test: 20 μL

Entrez Gene ID: 2208

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

341007 Rev. 1

Antibody Details

EBVCS-5

The CD23 antibody, clone EBVCS-5, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells from BALB/c mice immunized with an in vitro–transformed EBV cell line.

The CD23 antibody recognizes a 45-kilodalton (kDa) type II membrane glycoprotein, which is a human B-lymphocyte differentiation antigen. The CD23 antigen is also known as the low affinity IgE receptor, Fc epsilon RII, and FcεRII.

341007 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

341007 Rev.1

Citations & References

View product citations for antibody "341007" on CiteAb

Development References (14)

Capron M, Jouault T, Prin L, et al. Functional study of a monoclonal antibody to IgE Fc receptor (Fc epsilon R2) of eosinophils, platelets, and macrophages.. J Exp Med. 1986; 164(1):72-89. (Biology). View Reference
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Gordon J, Rowe M, Walker L, Guy G. Ligation of the CD23,p45 (Blast-2, EBVCS) antigen triggers the cell-cycle progression of activated B lymphocytes. Eur J Immunol. 1986; 16:1075-1080. (Biology).
Gordon J, Webb AJ, Walker L, Guy GR, Rowe M. Evidence of an association between CD23 and the receptor for a low molecular weight B cell growth factor. Eur J Immunol. 1986; 16:1627-1630. (Biology).
Kikutani H, Inui S, Sato R, et al. Molecular structure of human lymphocyte receptor for immunoglobulin E.. Cell. 1986; 47(5):657-65. (Biology). View Reference
Kikutani H, Suemura M, Owaki H, et al. Fc epsilon receptor, a specific differentiation marker transiently expressed on mature B cells before isotype switching.. J Exp Med. 1986; 164(5):1455-69. (Biology). View Reference
Kintner C, Sugden B. Identification of antigenic determinants unique to the surfaces of cells transformed by Epstein-Barr Virus. Nature. 1981; 294:458-460. (Biology).
Kwon HS, Park MC, Kim DG, et al. Identification of CD23 as a functional receptor for the proinflammatory cytokine AIMP1/p43. J Cell Sci. 2012; 125:4620-4629. (Biology).
Nadler LM. Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York, NY: Springer-Verlag; 1986:25-26.
Sherr E, Macy E, Kimata H, Gilly M, Saxon A. Binding the low affinity Fc ε R on B cells suppresses ongoing human IgE synthesis. J Immunol. 1989; 142:481-489. (Biology).
Thorley-Lawson D, Nadler L, Bhan A, Schooley R. Blast-2 (EBVCS), an early cell surface marker of human B cell activation, is superinduced by Epstein Barr Virus. J Immunol. 1985; 134:3007-3012. (Biology).
Yukawa K, Kikutani H, Owaki H, et al. A B-cell– specific differentiation antigen, CD23, is a receptor for IgE (FcεR) on lymphocytes. J Immunol. 1987; 138:2576-2580. (Biology).
van Dongen JJ, Lhermitte L, Böttcher S, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012; 26(9):1908-1975. (Biology). View Reference

View All (14)

341007 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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