Preparation And Storage
The monoclonal antibody is supplied as 10 µg purified immunoglobulin in 2.0 mL (5 µg/mL) of PBS. The FITC conjugate is supplied as 20 µg in 2.0 mL (10 µg/mL) of PBS. The PE conjugate is supplied as 3 µg in 1.0 mL (3 µg/mL) of PBS. The PE-Cy7 conjugate is supplied as 6.25 µg in 0.5 mL (12.5 µg/mL) of PBS. The APC conjugate is supplied as 6 µg in 0.5 mL (12 µg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.
Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
The CD25 antibody, clone 2A3. is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with PHA-activated human T lymphocytes. The CD25 antibody recognizes a 55-kilodalton (kDa) type I integral membrane glycoprotein. The CD25 antigen is also known as the human low-affinity interleukin-2 receptor α chain (IL-2Rα) and the Tac antigen.
Development References (13)
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Lando Z, Sarin P, Megson M, et al. Association of human T-cell leukaemia/lymphoma virus with the Tac antigen marker for the human T-cell growth factor receptor. Nature. 1983; 305(5936):733-736. (Biology). View Reference
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Ng WF, Duggan PJ, Ponchel F, et al. Human CD4(+)CD25(+) cells: a naturally occurring population of regulatory T cells. Blood. 2001; 98(9):2736-2744. (Biology). View Reference
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Robb RJ, Greene WC, Rusk CM. Low and high affinity cellular receptors for interleukin 2. Implications for the level of Tac antigen. J Exp Med. 1984; 160(4):1126-1146. (Biology). View Reference
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Urdal DL, March CJ, Gillis S, Larsen A, Dower SK. Purification and chemical characterization of the receptor for interleukin 2 from activated human T lymphocytes and from a human T-cell lymphoma cell line. Proc Natl Acad Sci U S A. 1984; 81(20):6481-6485. (Biology). View Reference
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