Skip to main content Skip to navigation
Hamburger Menu
Search icon
Order lookup icon Order lookup icon
Order Lookup
Country Selector Country Selector
United States (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Purified NA/LE Hamster Anti-Mouse CD40
      Purified NA/LE Hamster Anti-Mouse CD40
      Two-color analysis of the expression of CD40 on mouse spleen cells. BALB/c splenocytes were simultaneously stained with PE Rat Anti-Mouse CD45R/B220 (Cat. No. 553089/553090) and Purified NA/LE Hamster Anti-Mouse CD40 (Cat. No. 553721; right panel), followed by FITC Mouse Anti-Armenian Hamster IgM (Cat. No. 553721). Two-color contour plots were derived from gated events with the side and forward light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
      Purified NA/LE Hamster Anti-Mouse CD40
      Two-color analysis of the expression of CD40 on mouse spleen cells. BALB/c splenocytes were simultaneously stained with PE Rat Anti-Mouse CD45R/B220 (Cat. No. 553089/553090) and Purified NA/LE Hamster Anti-Mouse CD40 (Cat. No. 553721; right panel), followed by FITC Mouse Anti-Armenian Hamster IgM (Cat. No. 553721). Two-color contour plots were derived from gated events with the side and forward light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      Bp50; Tnfrsf5; TNR5; TRAP; CD40L receptor; GP39; HIGM1; IMD3; T-BAM
      Mouse (QC Testing), Rat (Tested in Development)
      Armenian Hamster IgM, κ
      (BALB/c x NZB) F1 Mouse-derived Lymphoma WEHI-231
      Flow cytometry (Routinely Tested), (Co)-stimulation, Blocking (Reported)
      1.0 mg/ml
      AB_395006
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.1 EU/µg (≤0.01 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      For immunohistochemical staining of mouse tissue, we recommend the use Purified Rat Anti-Mouse CD40 (clone 3/23; Cat. No. 550285) in our special formulation for immunohistochemistry.

      Note: This product may appear to contain aggregation and/or precipitation of the IgM antibody. Investigators are advised to briefly spin down any

      particulate matter.

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
      4. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      Show More blue down arrow Show Less blue up arrow
      553721 Rev. 3
      Antibody Details
      Down Arrow Up Arrow
      HM40-3

      The HM40-3 antibody reacts with CD40, a 40-50-kDa glycoprotein expressed on B lymphocytes and other antigen-presenting cells. The CD40 molecule has a central role in B-cell growth and differentiation. Furthermore, interactions of CD40 with its ligand, CD154, are involved in the initiation and effector stages of cell-mediated immune responses. CD40 may be involved in the triggering of NK cells and NK-T cells. Soluble HM40-3 antibody stimulates splenic and peritoneal B cells to proliferate in vitro. This antibody also induces spleen B cells to express the costimulatory molecules CD80 (B7-1) and CD86 (B7-2). HM40-3 mAb has been demonstrated to inhibit the binding of soluble CD154 (gp39, CD40 Ligand) to soluble CD40 and to cell-surface CD40. This hamster mAb to a mouse leukocyte antigen has been observed to cross-react with similar populations of Lewis, Sprague-Dawley, and LOU16 rat leukocytes.

      553721 Rev. 3
      Format Details
      Down Arrow Up Arrow
      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      553721 Rev.3
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "553721" on CiteAb

      Development References (16)

      1. Akiba H, Oshima H, Takeda K, et al. CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells. J Immunol. 1999; 162(12):7058-7066. (Clone-specific: (Co)-stimulation). View Reference
      2. Foy TM, Laman JD, Ledbetter JA, Aruffo A, Claassen E, Noelle RJ. gp39-CD40 interactions are essential for germinal center formation and the development of B cell memory. J Exp Med. 1994; 180(1):157-163. (Biology). View Reference
      3. Grewal IS, Flavell RA. CD40 and CD154 in cell-mediated immunity. Annu Rev Immunol. 1998; 16:111-135. (Biology). View Reference
      4. Inaba K, Witmer-Pack M, Inaba M, et al. The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro. J Exp Med. 1994; 180(5):1849-1860. (Immunogen). View Reference
      5. Inaba M, Inaba K, Fukuba Y, et al. Activation of thymic B cells by signals of CD40 molecules plus interleukin-10. Eur J Immunol. 1995; 25(5):1244-1248. (Clone-specific: (Co)-stimulation). View Reference
      6. Kaneko Y, Hirose S, Abe M, Yagita H, Okumura K, Shirai T. CD40-mediated stimulation of B1 and B2 cells: implication in autoantibody production in murine lupus. Eur J Immunol. 1996; 26(12):3061-3065. (Immunogen: (Co)-stimulation). View Reference
      7. Kashiwada M, Kaneko Y, Yagita H, Okumura K, Takemori T. Activation of mitogen-activated protein kinases via CD40 is distinct from that stimulated by surface IgM on B cells. Eur J Immunol. 1996; 26(7):1451-1458. (Clone-specific: (Co)-stimulation). View Reference
      8. Kawano T, Cui J, Koezuka Y, et al. CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides. Science. 1997; 278(5343):1626-1629. (Clone-specific: Blocking). View Reference
      9. Leifeld L, Trautwein C, Dumoulin FL, Manns MP, Sauerbruch T, Spengler U. Enhanced expression of CD80 (B7-1), CD86 (B7-2), and CD40 and their ligands CD28 and CD154 in fulminant hepatic failure. Am J Pathol. 1999; 154(6):1711-1720. (Biology). View Reference
      10. Munder M, Mallo M, Eichmann K, Modolell M. Murine macrophages secrete interferon gamma upon combined stimulation with interleukin (IL)-12 and IL-18: A novel pathway of autocrine macrophage activation. J Exp Med. 1998; 187(12):2103-2108. (Biology). View Reference
      11. Noelle RJ, Ledbetter JA, Aruffo A. CD40 and its ligand, an essential ligand-receptor pair for thymus-dependent B-cell activation. Immunol Today. 1992; 13(11):431-433. (Biology). View Reference
      12. Parry SL, Hasbold J, Holman M, Klaus GG. Hypercross-linking surface IgM or IgD receptors on mature B cells induces apoptosis that is reversed by costimulation with IL-4 and anti-CD40. J Immunol. 1994; 152(6):2821-2829. (Biology). View Reference
      13. Ridge JP, Di Rosa F, Matzinger P. A conditioned dendritic cell can be a temporal bridge between a CD4+ T-helper and a T-killer cell. Nature. 1998; 393(6684):474-478. (Clone-specific: (Co)-stimulation). View Reference
      14. Tomura M, Yu WG, Ahn HJ, et al. A novel function of Valpha14+CD4+NKT cells: stimulation of IL-12 production by antigen-presenting cells in the innate immune system. J Immunol. 1999; 163(1):93-101. (Biology). View Reference
      15. Trinite B, Voisine C, Yagita H, Josien R. A subset of cytolytic dendritic cells in rat. J Immunol. 2000; 165(8):4202-4208. (Biology). View Reference
      16. Turner JG, Rakhmilevich AL, Burdelya L, et al. Anti-CD40 antibody induces antitumor and antimetastatic effects: the role of NK cells. J Immunol. 2001; 166(1):89-94. (Biology). View Reference
      View All (16) View Less
      553721 Rev. 3

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.