CD117 BV605

BD Horizon™ CD117 BV605

Name: CD117 BV605
Brand: BD Horizon™
SKU: 663803

Clone 104D2

(ASR)

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Product Details

Brand: BD Horizon™

Alternative Name: KIT; c-Kit; SCFR; PBT; Mast/stem cell growth factor receptor

Reactivity: Human

Isotype: Mouse BALB/c IgG1

Immunogen: Megakaryoctic cell line MOLM-1

Application: Flow cytometry

Concentration: 50 μg/mL

Vol. Per Test: 5 μL

Workshop Number: VI C30

Entrez Gene ID: 3815

Regulatory Status: ASR

Product Notices

Caution: If you choose to combine two or more BD Horizon Brilliant™ Dyes in the same multicolor staining cocktail, fluorescent dye interactions may cause staining artifacts without the use of a buffering solution, such as the BD Horizon™ Brilliant Stain Buffer.

663803 Rev. 1

Antibody Details

104D2

The CD117 antibody, clone 104D2, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with the megakaryocytic cell line MOLM-1.

The CD117 antibody binds to a 145-kilodalton (kDa) type I transmembrane glycoprotein in the receptor tyrosine kinase (RTK) family. The CD117 antigen is also known as c-kit and stem cell factor receptor (SCFR).

663803 Rev. 1

Format Details

BV605

The BD Horizon Brilliant Violet™ 605 (BV605) dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 605-nm. BV605, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 610-nm (e.g., a 610/20-nm bandpass filter). The acceptor dye can be excited by the yellow-green (561-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: BV605

Excitation Source: Violet 405 nm

Excitation Max: 407 nm

Emission Max: 605 nm

663803 Rev.1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

Analyte Specific Reagent. Analytical and performance characteristics are not established.