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    Purified Rat Anti-Mouse Follicular Dendritic Cell
    Purified Rat Anti-Mouse Follicular Dendritic Cell
    Immunohistochemistry of follicular  dendritic cells. Frozen sections of mouse spleen was reacted with the FDC-M1 antibody. Follicular dendritic cells can be identified by the intense brown labeling of their cell membranes.  Magnification 20X.
    Purified Rat Anti-Mouse Follicular Dendritic Cell
    Immunohistochemistry of follicular  dendritic cells. Frozen sections of mouse spleen was reacted with the FDC-M1 antibody. Follicular dendritic cells can be identified by the intense brown labeling of their cell membranes.  Magnification 20X.
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    BD Pharmingen™
    Mouse (QC Testing)
    Rat IgG2c, κ
    Immunohistochemistry-frozen (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
    AB_394151
    Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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    Recommended Assay Procedures

    Immunohistochemistry: The FDC-M1 clone reactive against mouse follicular dendritic cells is tested for immunohistochemical staining of acetone-fixed frozen sections. Tissues tested were mouse spleen, thymus and small intestine. The antibody stains follicular dendritic cells and their processes. For optimal indirect immunohistochemical staining, the FDC-M1 antibody should be titrated (1-10 to 1-50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-rat Igs (multiple adsorbed) (Cat. No. 559286) as the secondary antibody and  treptravidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). More conveniently, the anti-rat Ig HRP detection kit (Cat. No. 551013) that provides the biotinylated secondary antibody, antibody diluent and the detection system can be used to stain for anti-mouse follicular dendritic cells. Adetailed protocol of the immunohistochemical procedure is enclosed. The clone FDC-M1 is not recommended for formalin-fixed paraffin embedded sections.

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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    551320 Rev. 1
    Antibody Details
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    FDC-M1

    Follicular Dendritic Cells (FDC) form a structural network within the lymphoid follicles and are involved in antigen presentation to B cells for generating an immune response and for the maintenance of immunological memory.  FDCs are believed to provide signals to B cells to induce their proliferation.  In LCMV mediated immunosuppression there is evidence of T-cell dependent destruction of macrophages and follicular dendritic cells.  The FDC-M1 antibody recognizes follicular dendritic cells and their dendritic processes.

    551320 Rev. 1
    Format Details
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    Purified
    Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
    Purified
    551320 Rev.1
    Citations & References
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    View product citations for antibody "551320" on CiteAb

    Development References (3)

    1. Gray D, Kosco M, Stockinger B. Novel pathways of antigen presentation for the maintenance of memory. Int Immunol. 1991; 3(2):141-148. (Biology). View Reference
    2. Kosco, Pflugfelder, and Gray. Follicular dendritic cell-dependent adhesion and proliferation of B cells in vitro. . J Immunol. 1992; 148:2331-2339. (Biology). View Reference
    3. Odermatt B, Eppler M, Leist TP, Hengartner H, Zinkernagel RM. Virus-triggered acquired immunodeficiency by cytotoxic T-cell-dependent destruction of antigen-presenting cells and lymph follicle structure. Proc Natl Acad Sci U S A. 1991; 88(18):8252-8256. (Biology). View Reference
    551320 Rev. 1

     

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.