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      PE Rat Anti-Mouse Ly-6G
      PE Rat Anti-Mouse Ly-6G
      Expression of Ly-6G on bone-marrow leukocytes. C57BL/6 bone-marrow cell suspensions were stained with either PE Rat Anti-Mouse Ly-6G (Cat. No. 551461,  open histograms) or PE Rat IgG2a, κ Isotype Control (Cat. No. 553930, filled histograms), in the presence of Mouse Fc Block™ purified anti-mouse CD16/CD32 (Cat. No. 553141/553142). Non-viable leukocytes were excluded by staining with Propidium Iodide Staining Solution (Cat. No. 556463), and leukocyte subsets were distinguished by their light-scatter profiles. The left panel displays lymphoid and erythroid cells, and the right panel displays myeloid cells. Flow cytometry was performed on a FACSCalibur™ (BDIS, San Jose, CA).
      PE Rat Anti-Mouse Ly-6G
      Expression of Ly-6G on bone-marrow leukocytes. C57BL/6 bone-marrow cell suspensions were stained with either PE Rat Anti-Mouse Ly-6G (Cat. No. 551461,  open histograms) or PE Rat IgG2a, κ Isotype Control (Cat. No. 553930, filled histograms), in the presence of Mouse Fc Block™ purified anti-mouse CD16/CD32 (Cat. No. 553141/553142). Non-viable leukocytes were excluded by staining with Propidium Iodide Staining Solution (Cat. No. 556463), and leukocyte subsets were distinguished by their light-scatter profiles. The left panel displays lymphoid and erythroid cells, and the right panel displays myeloid cells. Flow cytometry was performed on a FACSCalibur™ (BDIS, San Jose, CA).
      商品详情
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      BD Pharmingen™
      Ly6g; Lymphocyte antigen 6G; Lymphocyte antigen 6 complex, locus G; Gr1
      Mouse (QC Testing)
      Rat LEW, also known as Lewis IgG2a, κ
      Ly-6G-transfected EL4J Cell Line
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_394208
      Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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      推荐的实验流程

      For flow cytometry of leukocyte suspensions, we recommend the use of Mouse Fc Block™ (purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142).

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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      551461 Rev. 2
      抗体详情
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      1A8

      The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa GPI-anchored protein. In the bone marrow, Ly6G is expressed on the majority of the largest cells, predominantly granulocytes, but not on lymphoid or erythroid cells.  In the periphery, it is expressed on granulocytes. The mAb RB6-8C5 recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.

      551461 Rev. 2
      格式详情
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      551461 Rev.2
      报价单和参考
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      View product citations for antibody "551461" on CiteAb

      研发参考 (1)

      1. Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993; 151(5):2399-2408. (Immunogen). 查看参考
      551461 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.